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KMID : 0881720170320040267
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 4 p.267 ~ p.274
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A Monitoring of Aflatoxins in Commercial Herbs for Food and Medicine
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Kim Sung-Dan
Kim Ae-Kyung
Lee Hyun-Kyung
Lee Sae-Ram
Lee Hee-Jin
Ryu Hoe-Jin
Lee Jeong-Mi
Yoo In-Sil
Jung Kweon
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Abstract
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This paper deals with the natural occurrence of total aflatoxins (B1, B2, G1, and G2) in commercial herbs for food and medicine. To monitor aflatoxins in commercial herbs for food and medicine not included in the specifications of Food Code, a total of 62 samples of 6 different herbs (Bombycis Corpus, Glycyrrhizae Radix et Rhizoma, Menthae Herba, Nelumbinis Semen, Polygalae Radix, Zizyphi Semen) were collected from Yangnyeong market in Seoul, Korea. The samples were treated by the immunoaffinity column clean-up method and quantified by high performance liquid chromatography (HPLC) with on-line post column photochemical derivatization (PHRED) and fluorescence detection (FLD). The analytical method for aflatoxins was validated by accuracy, precision and detection limits. The method showed recovery values in the 86.9~114.0% range and the values of percent coefficient of variaton (CV%) in the 0.9~9.8% range. The limits of detection (LOD) and quantitation (LOQ) in herb were ranged from 0.020 to 0.363 μg/kg and from 0.059 to 1.101 μg/kg, respectively. Of 62 samples analyzed, 6 semens (the original form of 2 Nelumbinis Semen and 2 Zizyphi Semen, the powder of 1 Nelumbinis Semen and 1 Zizyphi Semen) were aflatoxin positive. Aflatoxins B1 or B2 were detected in all positive samples, and the presence of aflatoxins G1 and G2 were not detected. The amount of total aflatoxins (B1, B2, G1, and G2) in the powder and original form of Nelumbinis Semen and Zizyphi Semen were observed around ND~21.8 μg/kg, which is not regulated presently in Korea. The 56 samples presented levels below the limits of detection and quantitation.
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KEYWORD
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Herbs, Aflatoxins , HPLC , PHRED
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